How to Prepare a 100g/L Glycerol Solution from an 85v/v Stock Solution

Glycerol, a glycerol solution of 85 v/v, is commonly used in various applications for its viscosity and preservative properties. However, you might find the need to prepare a 100g/L solution, a common concentration in many biochemical assays and formulations. This guide will walk you through the process step-by-step, ensuring you achieve the desired concentration accurately.

Understanding the Data

To prepare the 100g/L solution, it's crucial to understand the density and volume percentages involved. Here, we're dealing with a 1.26 g/mL density of glycerol at 25°C and an 85 v/v solution, which means 85 mL of glycerol is dissolved in 150 mL of solvent (normally water), making the total volume of the solution 235 mL.

Calculating the Required Volumes

The target is to prepare a 100g/L glycerol solution with a final volume of 300 mL. This means you need 30 g of glycerol for 300 mL of the solution. Given the density of glycerol (1.26 g/mL), 30 g of glycerol would occupy:

30 g / 1.26 g/mL 23.81 mL

Since you only have an 85 v/v solution of glycerol, we need to calculate the volume of this solution required to obtain 23.81 mL of pure glycerol:

23.81 mL / 0.85 28.012 mL

This means you need to measure out 28.012 mL of the 85 v/v glycerol stock solution and dilute it with water to a final volume of 300 mL.

Step-by-Step Procedure

Measure out 28.012 mL of the 85 v/v glycerol stock solution using a calibrated pipette or volumetric flask to ensure accuracy.

Transfer the measured glycerol solution to a clean, 300 mL volume container.

Add distilled water slowly until the final volume reaches 300 mL. You can use a burette or a calibrated pipette for this step to ensure the precise volume is achieved.

Mix the solution thoroughly to ensure the glycerol is evenly distributed throughout the volume.

Conclusion

By following the steps outlined above, you can successfully prepare a 100g/L glycerol solution from an 85 v/v stock solution. It is critical to maintain precision when measuring volumes, especially when dealing with small amounts of concentrated solutions.

Keywords: glycerol solution preparation, 85v/v glycerol, 100g/L glycerol